Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. 2. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Table of Contents. SpectraMax® ABS. KEY FEATURES • Five. 1. SpectraMax iD5 Multi-Mode Microplate Reader. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax® ABS Plus. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Bookings are free, but you will need to use iLab to book. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). , Sunnyvale, CA) using the bottom well-scan mode where nine readings. FlexStation® 3 Multi-Mode Microplate Reader. 0. 1 2. 6 for further experiment, so I'm confused which i would choose for OD value determination. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Shop now. 3 mg. Nontreated cells in DMEM medium were used as a control. All associated StakMax® stackers. Flexible for 6-well to 384-well microplates and cuvettes. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 00 USD. 1 second. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Choose from a three- (M3), four-. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Fifteen different automated tests are provided. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. When a. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. , Sunny-vale, CA). SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. • Tel. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Bookings are free, but you will need to use iLab to book. 5 Dual-luciferase reporter assay. AquaMax® 4000. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. 1** Version 7. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 2. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. The SpectraMax® ABS Plus Microplate Reader can run . Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The system. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email [email protected]% Triton X‐100, 10 mM potassium phosphate buffer pH 7. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. FlexStation™ III. Three independent experiments were performed. Plate chamber design of the SpectraMax category 1 microplate readers. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Published Dec 16, 2020 | Updated Dec 16, 2020. 2. 7°C and the bottom to 36. Devices’ StakMax® Microplate Stacker in a matter of minutes and . edu) to be added as a trained user in iLab. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). We would like to show you a description here but the site won’t allow us. Selection of Water Constant in PathCheck settings. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Or, with the reader specifications noted from the Instrument Description section of your reader's. The cells were incubated with compounds at the indicated doses for 1 h. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Figure 1. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Sensitive fluorescent quantitation of DNA with the Quant-iT. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. E Models Only) 69. The following document is intended to demonstrate setup of this instrument. SpectraMax M5 reader settings are shown. 1. 5°C at 37°C. Microplate Readers are modular and . Figure 1. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. SpectraMax 250 User. 2. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. Microplate Readers Include the M3, M4, M5, and M5. This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Microplate Reader 96 & 384 Thermo Multiskan Ascent w. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. ) by nonlinear regression using. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Turn on the microplate reader. 2. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax M Series multi-mode microplates are configurable readers with triple. 2. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Table of Contents. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. SDS-PAGE Assay. Has anyone had problems with the built-in computer of the Spectramax ID3. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 00. ,. Western blotting analysis. Category 2 and 3 microplate readers use gradient heating as well. The advantage of using labeled probes is detection specificity. 67. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Ltd. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 14 Average %CV 0. Recertification is recommended every two years. The assay window spanned four orders of magnitude. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Figure 4: Transcreener ADP. Page 1 of 8 . Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). 7 1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. Microplate Readers. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Filterless readers provide consistent results with steady temperature regulation. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 72 MB. 7 cells were co-transfected with 0. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Background fluorescence has been subtracted. Molecular Devices. Note: In this user guide, all references to SpectraMax Multi-Mode. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. All assays were performed in at least three individual experiments, each comprising no. Expanded wavelength coverage. Ferguson, Ph. PURPOSE. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 1. Description. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. Western blotting assays were conducted according to previous studies . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. book Page 7 Wednesday, July 7, 2010 2:09 PM. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. SpectraMax Glo Steady-Luc Reporter Assay Kit. ® reader to the computer. edu) to be added as a trained user in iLab. Equipped with scanning, shaking and kinetics modules. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates orThe optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. 73. When a feature or capability applies to only certain readers, this exception is noted. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Molecular Devices Corporation. It cannot be used for luminescence measurements. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. 2 only). Maximum signal with lowest background. Wide range of applications. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. . "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. 1 Version 7. Examples: “Press carefully to engage,” “ Do not. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. Choose from a. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. Depending on which. 6-384. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. All associated StakMax® stackers. 05% Tween 20 using a Nunc ELISA plate washer. 1. Make sure the microplate is flat against. 2. free tracer decreases, resulting in. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. To block unwanted residual excitation light and minimize background interference, a 570 nm. Tunable wavelength selection / monochromator. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Five preferred modes for diverse bioresearch applications. These robust readers have been placed in labs from Antarctica to the. 0 out of 5 $ 16,499. We use Spectramax M5 microplate reader in our lab. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 6 0. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Microplate Capacity. No additional manuals are available. Spectramax Multi-Mode Microplate Reader Performance Specifications. The Multiskan Sky microplate reader is an absorbance plate reader. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. The dual monochromator optics allow the widest range of applications to be. 0 out of 5 $ 2,499 $ 1,999. flexibility of monochromator-based systems. 26 1. In brief, RAW 264. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. 00 USD. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. com A. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Self Service Available. Molecular Devices SpectraMax M5 Multimode Microplate Reader. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax M2 and M2e are versatile and reliable. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. spectramax m5 multi. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Other technologies such as imaging. S. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Wide range of applications The VersaMax reader covers. Microplate formats. Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax® M4. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. The system. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. 313 reference gain check fail. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. M3-M5e_manual. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. For the Live/Dead assay, they were treated. The Spectr. com. All reagents were brought to room temperature before use. SpectraMax® iD5. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SpectraMax® ABS Plus. book Page 7 Wednesday, July 7, 2010 2:09 PM. RAW 264. 1. SoftMax Pro. Example: [Drawer]. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Here the delta mP was 336, and Z’ factor was 0. 59 6. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideFluorescence Microplate Reader Comparison Chart. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. Figure 1. Wide range of applications. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Microplate Readers include the M3, M4, M5, and M5e models. Five preferred detection modes. Microplate Readers include the M3, M4, M5, and M5e models. Or scan up to 384 wells at any time to determine the best assay conditions. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. 72 MB. Levels of immunoreactivity were normalized to. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Molecular Devices Spectramax M5 Microplate Reader; Related products. Lift up the lid over the chamber to insert or remove a cuvette. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Do not touch the clear surfaces of the cuvette. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). The following document is intended to demonstrate setup of this instrument. Introduction. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. The following document is. In . Add to Cart. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. The raw data from each individual experiment were normalized to vehicle-treated HK-2. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. Sale price $18,499. SpectraMax® ABS. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. com. (The i3x and Paradigm readers require the. FlexStation™ II. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. View the Molecular Devices website. l. This. The drawing shows a standard microplate of 15 mm height covered with a lid. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Are you using the correct Microplate Adapters? For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. FlexStation™ II. 6 for further experiment, so I'm confused which i would choose for OD value determination. RAW 264. 3 Version 7. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. both cuvette-based and microplate reader applications on the same instrument. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Introduction. SpectraMax M5 Multi-Mode Microplate Reader.