SpectraMax® M5e. 6 0. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Wide range of applications. SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Page 28 Chapter 5: Read Modes and Read Types 1. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Description. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. TR-FRET assay principle. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Table of Contents. 2. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Instrument settings are shown in Figure 3. View the Molecular Devices website. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Five preferred detection modes. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. 67. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. 2. Molecular Devices SpectraMax M5 Multimode Microplate Reader. The PBS solution (10. No additional manuals are available. 96-384. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Data are normalized to vehicle-treated. 1. 1 2. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The PBS solution (10. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. All assays were performed in at least three individual experiments, each comprising no. 6-384 (96- and 384-well with pipetting) 6-384. 49 2. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 96-384. (Shanghai, China). view more . Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. We use Spectramax M5 microplate reader in our lab. No accessories are needed and SoftMax® Pro Software. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 1. When a feature or capability applies to only certain readers, this exception is noted. 1. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Microplate Readers include the M3, M4,. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Maximum signal with lowest background. 6% for the on-board optics (monochromators). Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. Lift up the lid over the chamber to insert or remove a cuvette. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). SpectraMax 250 User. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 1** Version 7. 26 1. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. 0 . Between steps, reactions were rinsed 3 times with TBS + 0. 5 12. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. analysis, controls the SpectraMax Multi-Mode Microplate Readers. for ValitaTiter Plus standards read on Molecular Devices readers. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. For a higher . The cells were then treated to induce apoptosis. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Temperature variation ± 0. Figure 1. SpectraMax M5e Multi-Mode Microplate Reader. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. M3 M4 M5 M5e LM1 v<nnn>. 2 only). Five. Choose cables that meet the requirement described in this appendix. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. spectramax m5 multi. Tunable wavelength selection / monochromator. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Note: In this user guide, all references to SpectraMax Multi-Mode. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. , USA). It cannot be used for luminescence measurements. Fluorescence Microplate Readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Spectramax Multi-Mode Microplate Reader Performance Specifications. • Tel. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . , Sunnyvale, CA) using the bottom well-scan mode where nine readings. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . . 5 87. When a feature or capability applies to only certain readers, this exception is noted. D. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. 00 USD. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. This application note describes how to. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. Nontreated cells in DMEM medium were used as a control. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The drawing shows a standard microplate of 15 mm height covered with a lid. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader Can you recommend disposable cuvettes for use in the microplate readers? Molecular Devices® does not recommend any specific disposable cuvette brands. Other technologies such as imaging. All associated StakMax® stackers. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. Lower limits of detection for ATP determined using the ATPlite . Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. The guide also covers the software features, the specifications, and the accessories of the systems. . Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Add to Cart. M3-M5e_manual. 4. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). ATP standard curves read on SpectraMax microplate readers. 1 Version 7. Microplate formats. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. IMAP assays are based on binding of. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. book Page 7 Wednesday, July 7, 2010 2:09 PM. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. 2. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Self Service Available. Wide range of applications. 40266 Email: drugdiscoverytech@lifetech. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. 5 Dual-luciferase reporter assay. 2. Western blotting analysis. Background fluorescence has been subtracted. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Has anyone had problems with the built-in computer of the Spectramax ID3. 2. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Filterless readers provide consistent results with steady temperature regulation. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 00 USD. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). When a FlexStation® 3 Multi-Mode Microplate Reader. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. 0 out of 5 $ 17,999 $ 16,995. com. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. 3 mg. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). The calculated LOQ values were 0. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email [email protected]% Triton X‐100, 10 mM potassium phosphate buffer pH 7. Also refer to this Multimode Reader Comparison Chart . System Diagrams and Dimensions. AquaMax® 2000. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 4 Max StDev 1. Microplate Readers. Qualify the fluorescence performance of the SpectraMax . M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. We use Spectramax M5 microplate reader in our lab. Spectramax M5 microplate reader: Molecular Devices: N/A:. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Three independent experiments were performed. Flexible for 6-well to 384-well microplates and cuvettes. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Figure 4: Transcreener ADP. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Softmax Pro GxP Software 6. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 04 to 3. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. 7. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. The chamber contains springs that position the cuvette in the proper alignment for a read. Bottom-reading is usually recommended for cell-based assay with adherent cells. Fluorescence Microplate Reader Comparison Chart. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Microplate reader settings. The dual monochromator optics allow the widest. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. The chamber contains springs that position the cuvette in the proper alignment for a read. Call 516-939-0888 to check. When wells are assigned to template groups, data will populate group tables where analysis canThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Cables. Compact and tunable absorbance microplate readers DATA SHEET. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Download PDF. The following document is intended to demonstrate setup of this instrument. 6 for further experiment, so I'm confused which i would choose for OD value determination. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Example: [Drawer]. 4. At the end of this period, cells were exposed to FA (1 mM) for 1 h. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Introduction. free tracer decreases, resulting in. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. #M5) SpectraMax® L Microplate Reader (cat. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. 54 1. Methods Preparation of reagents and standards. Microplate Readers are modular and . Figure 3. Learn how you can utilize microplate readers to drive landmark discoveries. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Selection of Water Constant in PathCheck settings. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. HTRF-compatible SpectraMax Multi-Mode Readers. MOLECULAR DEVICES SpectraMax M5 also includes a wide selection of curve-fitting functions, allowing users to perform accurate and reliable quantification of biochemical reactions. . SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 2 only) SpectraMax M-series Microplate readers. Small, unbound molecules rotate rapidly in. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. 6 for further experiment, so I'm confused which i would choose for OD value determination. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Linear, Orbital Slow, and Orbital Fast. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The cuvette must be in place when you read the plate. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Label-free detection. Microplate Readers Biotek Synergy H1 Hybrid Microplate. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Qualify the luminescence performance of the SpectraMax . Microplate Readers include the M3, M4, M5, and M5e models. Compatible automation solutions for SpectraMax Readers. 7 1. 14 Average %CV 0. Wide range of applications. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The integration time suggested is 5-10 sec. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Example: [Drawer]. SpectraMax® iD5. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. When a feature or capability applies to only certain readers, this exception is noted. ® reader to the computer. Molecular Devices | Life Science Applications | Bioanalytical. 0. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. SpectraMax M Series multi-mode microplates are configurable readers with triple. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. com A. To block unwanted residual excitation light and minimize background interference, a 570 nm. Western blotting assays were conducted according to previous studies . ) by nonlinear regression using. 63 1. 05% Tween 20 using a Nunc ELISA plate washer. Microplate Reader Documentation Version No. Italic and boldface type are used for emphasis. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Are you using the correct Microplate Adapters? For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. book Page 7 Wednesday, July 7, 2010 2:09 PM. ,. Go to Product Details. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Five preferred detection modes. free tracer decreases, resulting in an. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Catalog Number: (89212-396) Supplier: Molecular Devices. Supplier: Molecular Devices. Add to Cart. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Learn how to set up protocols, customize reports, export results, and more with this PDF document. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. Molecular Devices. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. used. 96-1536. E Models Only) 69. 1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. reader in the visible range between 405–750 nm with NIST and NMI. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. com. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. You can use either. Figure 1. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 1. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. B: Selection of Excitation Wavelength. Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax M2 and M2e are versatile and reliable. This. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 6-384. The cuvette must be in place when you read the plate. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. keeping with best practices, reader performance should. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Published Dec 16, 2020 | Updated Dec 16, 2020. M3-M5e_manual. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Protocol Name Version 6. Add to Cart. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. The day before assay, assay buffer was prepared as described in the product technical manual. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 1 & 7. edu) to be added as a trained user in iLab. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. E Models. Fill a clean plate with water. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products.